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c1000 touchtm thermal cycler  (Bio-Rad)


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    Structured Review

    Bio-Rad c1000 touchtm thermal cycler
    C1000 Touchtm Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 7321 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c1000+touchtm+thermal+cycler/QX200+Droplet+Reader/pm42047179-92-7-11
    Average 98 stars, based on 7321 article reviews
    c1000 touchtm thermal cycler - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs
    Article Snippet: Droplets were generated using Automated Droplet Generation Oil for Probes (Bio-Rad) and DG32TM Automated Droplet Generator Cartridges with the automatic droplet generator (Bio-Rad AutoDG), and the plate sealed with a PCR Plate Heat Seal (Bio-Rad) and PX1TM PCR Plate Sealer (Bio-Rad). .. PCR was performed using the C1000 TouchTM Thermal Cycler with 96-Deep Well Reaction (Bio-Rad) and droplets read using a QX600 Droplet Reader (Bio-Rad). .. Droplet thresholds were manually set using QX Manager Software (Bio-Rad, Version 2.3) and processed in Excel (Microsoft).

    Article Title: Unveiling the phylogenetic position of the type species of the syndinean genus Amoebophrya and broad divergence of the A. ceratii complex.
    Article Snippet: .. All PCRs were run in a total reaction volume of 20 μL, containing 2 μL of template DNA, forward and reverse primers each at a final concentration of 0.3 μM, and deionized sterile water, using an AccuPower® PCR premix (Bioneer Inc., Daejeon, Korea) and by using a C1000 TouchTM thermal cycler (Bio- Rad Laboratories, Inc., Hercules, California, United States) under the following conditions: an initial denaturing step at 95°C for 3 min followed by 35 cycles of 95°C for 45 s, 50–55°C for 45 s, and 72°C for 1 min and with a final extension at 72°C for 7 min. Amplicons were treated using ExoSAP- ITTM Express PCR Product Cleanup (Applied Biosystems, Thermo Fisher Scientific, California, United States) prior to sequencing. .. DNA sequencing was performed with the same primer sets as those used for PCR amplification for each target region (18S rRNA gene, ITS15.8S- ITS2 rRNA region, and 28S rRNA gene) on an ABI model 3730xl DNA Analyzer (Applied Biosystems, Thermo Fisher Scientific, California, United States).

    Article Title: Clinical evaluation of a validated droplet digital PCR assay for the detection of Ehrlichia canis in ocular and blood specimens from seroreactive dogs.
    Article Snippet: Limited data are available on the diagnostic value of ocular specimens in Ehrlichia canis-infected dogs.. This study evaluated the diagnostic sensitivity of conjunctival swabs compared with whole blood in dogs clinically suspected of canine monocytic ehrlichiosis (CME).. Forty-one seropositive dogs (as determined by the IDEXX SNAP 4Dx Plus test) exhibiting clinical signs consistent with CME were enrolled.

    Article Title: DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs.
    Article Snippet: Droplets were generated using Automated Droplet Generation Oil for Probes (BioRad) and DG32TM Automated Droplet Generator Cartridges with the automatic droplet generator (Bio-Rad AutoDG), and the plate sealed with a PCR Plate Heat Seal (Bio-Rad) and PX1TM PCR Plate Sealer (Bio-Rad). .. PCR was performed using the C1000 TouchTM Thermal Cycler with 96-Deep Well Reaction (Bio-Rad) and droplets read using a QX600 Droplet Reader (Bio-Rad). .. Droplet thresholds were manually set using QX Manager Software (Bio-Rad, Version 2.3) and processed in Excel (Microsoft).

    Article Title: Development and evaluation of quadruplex droplet digital PCR assay for rapid detection of molecular markers associated with macrocyclic lactone resistance and susceptibility in Dirofilaria immitis
    Article Snippet: .. The PCR was performed on a C1000 TouchTM thermal cycler (Bio-Rad). .. Fluorescence signals were measured using the QX200TM Droplet Reader (Bio-Rad), and target concentrations were determined in copies/μl using Poisson's statistics.

    Article Title: Longitudinal circulating tumor DNA profiling in patients with advanced endometrial cancer using an off-the-shelf targeted NGS panel.
    Article Snippet: PCR was performed using Bio-Rad’s ddPCR Supermix and samples were partitioned into ~50 000 droplets across triplicates using an automated droplet generator (Bio-Rad Laboratories, Hercules, CA, USA). .. Emulsified PCR reactions were run on a C1000 TouchTM thermal cycler (Bio-Rad Laboratories), and results were analyzed with a Bio-Rad QX-200 droplet reader (Bio-Rad Laboratories) and QuantaSoft v1.7.4 software (Bio-Rad Laboratories). ..

    Concentration Assay:

    Article Title: Unveiling the phylogenetic position of the type species of the syndinean genus Amoebophrya and broad divergence of the A. ceratii complex.
    Article Snippet: .. All PCRs were run in a total reaction volume of 20 μL, containing 2 μL of template DNA, forward and reverse primers each at a final concentration of 0.3 μM, and deionized sterile water, using an AccuPower® PCR premix (Bioneer Inc., Daejeon, Korea) and by using a C1000 TouchTM thermal cycler (Bio- Rad Laboratories, Inc., Hercules, California, United States) under the following conditions: an initial denaturing step at 95°C for 3 min followed by 35 cycles of 95°C for 45 s, 50–55°C for 45 s, and 72°C for 1 min and with a final extension at 72°C for 7 min. Amplicons were treated using ExoSAP- ITTM Express PCR Product Cleanup (Applied Biosystems, Thermo Fisher Scientific, California, United States) prior to sequencing. .. DNA sequencing was performed with the same primer sets as those used for PCR amplification for each target region (18S rRNA gene, ITS15.8S- ITS2 rRNA region, and 28S rRNA gene) on an ABI model 3730xl DNA Analyzer (Applied Biosystems, Thermo Fisher Scientific, California, United States).

    Sterility:

    Article Title: Unveiling the phylogenetic position of the type species of the syndinean genus Amoebophrya and broad divergence of the A. ceratii complex.
    Article Snippet: .. All PCRs were run in a total reaction volume of 20 μL, containing 2 μL of template DNA, forward and reverse primers each at a final concentration of 0.3 μM, and deionized sterile water, using an AccuPower® PCR premix (Bioneer Inc., Daejeon, Korea) and by using a C1000 TouchTM thermal cycler (Bio- Rad Laboratories, Inc., Hercules, California, United States) under the following conditions: an initial denaturing step at 95°C for 3 min followed by 35 cycles of 95°C for 45 s, 50–55°C for 45 s, and 72°C for 1 min and with a final extension at 72°C for 7 min. Amplicons were treated using ExoSAP- ITTM Express PCR Product Cleanup (Applied Biosystems, Thermo Fisher Scientific, California, United States) prior to sequencing. .. DNA sequencing was performed with the same primer sets as those used for PCR amplification for each target region (18S rRNA gene, ITS15.8S- ITS2 rRNA region, and 28S rRNA gene) on an ABI model 3730xl DNA Analyzer (Applied Biosystems, Thermo Fisher Scientific, California, United States).

    Sequencing:

    Article Title: Unveiling the phylogenetic position of the type species of the syndinean genus Amoebophrya and broad divergence of the A. ceratii complex.
    Article Snippet: .. All PCRs were run in a total reaction volume of 20 μL, containing 2 μL of template DNA, forward and reverse primers each at a final concentration of 0.3 μM, and deionized sterile water, using an AccuPower® PCR premix (Bioneer Inc., Daejeon, Korea) and by using a C1000 TouchTM thermal cycler (Bio- Rad Laboratories, Inc., Hercules, California, United States) under the following conditions: an initial denaturing step at 95°C for 3 min followed by 35 cycles of 95°C for 45 s, 50–55°C for 45 s, and 72°C for 1 min and with a final extension at 72°C for 7 min. Amplicons were treated using ExoSAP- ITTM Express PCR Product Cleanup (Applied Biosystems, Thermo Fisher Scientific, California, United States) prior to sequencing. .. DNA sequencing was performed with the same primer sets as those used for PCR amplification for each target region (18S rRNA gene, ITS15.8S- ITS2 rRNA region, and 28S rRNA gene) on an ABI model 3730xl DNA Analyzer (Applied Biosystems, Thermo Fisher Scientific, California, United States).

    Synthesized:

    Article Title: Meis1 Negatively Regulates Epithelial-Mesenchymal Transition via Wnt/β-catenin Pathway in Oral Submucous Fibrosis
    Article Snippet: Total RNA was extracted from cells using TRIzol (TIANGEN, DP424). .. Complementary DNA (cDNA) was synthesized by reverse transcription using the same kit (TIANGEN, DP424) according to the manufacturer’s instructions. qRT-PCR was performed on a C1000 TouchTM thermal cycler (Bio-Rad) with SYBR Green mixture (Bio-Rad). ..

    Reverse Transcription:

    Article Title: Meis1 Negatively Regulates Epithelial-Mesenchymal Transition via Wnt/β-catenin Pathway in Oral Submucous Fibrosis
    Article Snippet: Total RNA was extracted from cells using TRIzol (TIANGEN, DP424). .. Complementary DNA (cDNA) was synthesized by reverse transcription using the same kit (TIANGEN, DP424) according to the manufacturer’s instructions. qRT-PCR was performed on a C1000 TouchTM thermal cycler (Bio-Rad) with SYBR Green mixture (Bio-Rad). ..

    Quantitative RT-PCR:

    Article Title: Meis1 Negatively Regulates Epithelial-Mesenchymal Transition via Wnt/β-catenin Pathway in Oral Submucous Fibrosis
    Article Snippet: Total RNA was extracted from cells using TRIzol (TIANGEN, DP424). .. Complementary DNA (cDNA) was synthesized by reverse transcription using the same kit (TIANGEN, DP424) according to the manufacturer’s instructions. qRT-PCR was performed on a C1000 TouchTM thermal cycler (Bio-Rad) with SYBR Green mixture (Bio-Rad). ..

    SYBR Green Assay:

    Article Title: Meis1 Negatively Regulates Epithelial-Mesenchymal Transition via Wnt/β-catenin Pathway in Oral Submucous Fibrosis
    Article Snippet: Total RNA was extracted from cells using TRIzol (TIANGEN, DP424). .. Complementary DNA (cDNA) was synthesized by reverse transcription using the same kit (TIANGEN, DP424) according to the manufacturer’s instructions. qRT-PCR was performed on a C1000 TouchTM thermal cycler (Bio-Rad) with SYBR Green mixture (Bio-Rad). ..

    Amplification:

    Article Title: Clinical evaluation of a validated droplet digital PCR assay for the detection of Ehrlichia canis in ocular and blood specimens from seroreactive dogs.
    Article Snippet: Limited data are available on the diagnostic value of ocular specimens in Ehrlichia canis-infected dogs.. This study evaluated the diagnostic sensitivity of conjunctival swabs compared with whole blood in dogs clinically suspected of canine monocytic ehrlichiosis (CME).. Forty-one seropositive dogs (as determined by the IDEXX SNAP 4Dx Plus test) exhibiting clinical signs consistent with CME were enrolled.

    Software:

    Article Title: Longitudinal circulating tumor DNA profiling in patients with advanced endometrial cancer using an off-the-shelf targeted NGS panel.
    Article Snippet: PCR was performed using Bio-Rad’s ddPCR Supermix and samples were partitioned into ~50 000 droplets across triplicates using an automated droplet generator (Bio-Rad Laboratories, Hercules, CA, USA). .. Emulsified PCR reactions were run on a C1000 TouchTM thermal cycler (Bio-Rad Laboratories), and results were analyzed with a Bio-Rad QX-200 droplet reader (Bio-Rad Laboratories) and QuantaSoft v1.7.4 software (Bio-Rad Laboratories). ..

    Generated:

    Article Title: Investigation of cyanobacteria-hosted antibiotic resistance genes in cyanoHAB-impacted drinking water sources.
    Article Snippet: .. These genes were selected because of widespread tetracycline and sulfonamide antibiotic use in healthcare and agriculture (Baran et al. 2011; Daghrir & Drogui 2013) and MGE as a transporter of ARGs (Ghaly & Gillings 2021). ddPCR droplets were generated using the QX200 droplet generator (Bio-Rad), and thermal cycling was conducted with the C1000 TouchTM Thermal Cycler (Bio-Rad, Hercules, CA, USA). .. Gene copies were quantified using the QX200 Droplet Reader and QuantaSoft Software (Bio-Rad, Hercules, CA, USA), and a positive and negative control were included with every run.



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